Biofilm rigidity, mechanics and composition in seawater desalination pretreatment employing ultrafiltration and microfiltration membranes.

The choice of appropriate biofilm control strategies in membrane systems for seawater desalination pretreatment relies on understanding the properties of the biofilm formed on the membrane. This study reveals how the biofilm composition, including both organic and inorganic, influenced the biofilm behavior under mechanical loading. The investigation was conducted on two Gravity-Driven Membrane reactors employing Microfiltration (MF) and Ultrafiltration (UF) membrane for the pretreatment of raw seawater. After a stabilization period of 20 days (Phase I), a biofilm behavior test was introduced (Phase II) to evaluate (i) biofilm deformation during the absence of permeation (i.e., relaxation) and (ii) biofilm resistance to detachment forces (i.e., air scouring). The in-situ monitoring investigation using Optical Coherence Tomography (OCT) revealed that the biofilms developed on MF and UF membrane presented a rigid structure in absence of filtration forces, limiting the application of relaxation and biofilm expansion necessary for cleaning. Moreover, under shear stress conditions, a higher reduction in biofilm thickness was observed for MF (-60%, from 84 to 34 µm) compared to UF (-30%, from 64 to 45 µm), leading to an increase of permeate flux (+60%, from 9.1 to 14.9 L/m2/h and +20 % from 7.8 to 9.5 L/m2/h, respectively). The rheometric analysis indicated that the biofilm developed on MF membrane had weaker mechanical strength, displaying lower storage modulus (-50 %) and lower loss modulus (-55 %) compared to UF. These differences in mechanical properties were linked to the lower concentration of polyvalent ions and the distribution of organic foulants (i.e., BB, LMW-N) found in the biofilm on the MF membrane. Moreover, in the presence of air scouring led to a slight difference in microbial community between UF and MF. Our findings provide valuable insight for future investigations aimed at engineer biofilm composition to optimize biofilm control strategies in membrane systems for seawater desalination pretreatment.

Periodic fouling control strategies in gravity-driven membrane bioreactors (GD-MBRs): Impact on treatment performance and membrane fouling properties.

This study aims to assess the effects of periodic membrane fouling control strategies in Gravity-Driven Membrane Bioreactor (GD-MBR) treating primary wastewater. The impact of each control strategy on the reactor performance (permeate flux and water quality), biomass morphology, and fouling composition were evaluated. The application of air scouring coupled with intermittent filtration resulted in the highest permeate flux (4 LMH) compared to only intermittent filtration (i.e., relaxation) (1 LMH) and air scouring under continuous filtration (2.5 LMH). Air scouring coupled with relaxation led to a thin (~50 µm) but with more porous fouling layer and low hydraulic resistance, presenting the lowest concentration of extracellular polymeric substance (EPS) in the biomass. Air scouring under continuous filtration led to a thin (~50 µm), dense, compact, and less porous fouling layer with the highest specific hydraulic resistance. The employment of only relaxation led to the highest fouling formation (~280 µm) on the membrane surface. The highest TN removal (~62%) was achieved in the reactor with only relaxation (no aeration) due to the anoxic condition in the filtration tank, while the highest COD removal (~ 60%) was achieved with air scouring under continuous filtration due to the longer aeration time and the denser fouling layer. The results highlighted the importance of performing in-depth fouling characterization to link the membrane fouling properties to the hydraulic resistance and membrane bioreactor performances (i.e., water quality and water production). Moreover, this work proven the versatility of the GD-MBR, where the choice of the appropriate operation and fouling control strategy relies on the eventual discharge or reuse of the treated effluent.

Aquarium-borne Mycobacterium marinum skin infection. Report of 15 cases and review of the literature.

Mycobacterium marinum is a non-tuberculous photochromogenic mycobacterium, commonly responsible for fish and amphibious infections world-wide. Contagion in humans typically follows minor hand trauma from aquarium keeping and manifests as a granulomatous infection of the skin. Dissemination is rare and almost exclusive to immunosuppressed hosts. 15 cases of M. marinum fish tank related infection are hereby reported. The site of infection was the upper limbs in all cases. 3 patients presented a single papulo-verrucous lesion, while the remaining 12 showed a sporotrichoid clinical pattern. Diagnosis was reached by history and clinical examination and further supported by one or more of the following criteria: histology, culture, acid fast bacilli identification from histologic specimen and PCR. 2 to 3 months minocycline treatment showed efficacy in 13 individuals, another case was treated with rifampicin-isoniazid association, yet another showed spontaneous regression over a 3 month period.

Nummular eczema and contact allergy: a retrospective study.

BACKGROUND: Etiopathogenesis of nummular eczema is obscure; many causative factors have been proposed. Only a few studies investigated the relevance of contact allergy. OBJECTIVE: This retrospective study aimed to investigate the role of contact allergy in the underlying mechanism of nummular eczema. METHODS: From the 29,323 consecutive patients, we patch-tested for eczematous dermatitis of various type, 1022 (3.5%) with nummular eczema were enrolled. Data were collected for each patient, including age, sex, occupation, symptoms onset and duration, onset and spread sites, and clinical evidence or history of atopy. Histological analyses of acute phase lesions were carried out in some patients. RESULTS: Peak incidence of age at disease onset was found in the third decade of life. Predominant sites of lesions were upper (75.8%) and lower (64.5%) limbs, followed by trunk, dorsum of the hands, and face and neck. Three hundred thirty-two (32.5%) of 1022 patients showed positive reactions to 1 or more allergens. Highest sensitization rates were found with nickel sulfate (10.2%), potassium dichromate (7.3%), and cobalt chloride (6.1%). Histopathology showed less pronounced spongiosis in atopic subjects and the elderly. CONCLUSIONS: Because this study demonstrates that contact allergy is common with nummular eczema, patch testing is strongly advisable in every patient with persistent nummular dermatitis.